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The Alphasil of high-performance liquid chromatographic (HPLC) columns employ ultrapure spherical silica gel as the matrix, leveraging unique silica gel surface bonding technologies such as "polar copolymerization," "click chemistry," and deep end-capping techniques. These columns exhibit outstanding chromatographic performance in terms of selectivity, peak shape, and stability. Furthermore, our company has established rigorous production process control requirements and corresponding quality standards to ensure stable product properties and exceptional durability. This comprehensive series boasts a full range of bonded phases, covering reverse-phase chromatography, hydrophilic interaction chromatography, and normal-phase chromatography products, catering to the separation and analysis of weakly polar, moderately polar, and strongly polar compounds alike. This versatility provides users with flexible options for method development.

Product Types Include:

Reverse-Phase Chromatographic Columns:

· Alphasil ES-C18

· Alphasil VC-C18

· Alphasil VC-C18AQ

· Alphasil XD-C18AQ

· Alphasil XD-C18CH

· Alphasil XD-Ph

· Alphasil S-PFP

· Alphasil S-AC Pigment-Specific Column

· Alphasil XAqua C18 Calculus Bovis Column

Hydrophilic Interaction Columns / Normal-Phase Columns:

· Alphasil VC-HILIC

· Alphasil S-Xion

Alphasil ES Columns

The Alphasil ES-C18 utilizes sterically protected bonding technology, enabling excellent stability and prolonged column life within a pH range of 0.8-8. This makes it an ideal choice for low-pH method development. Its proprietary peak tailing technology effectively reduces the activity of surface silanol groups, delivering exceptional peak shapes for acidic, basic, and neutral compounds.

Alphasil VC Columns

The Alphasil VC-C18 column represents a classic high-performance reverse-phase column, suitable for the analysis of non-polar to moderately polar acidic, basic, and neutral compounds, making it ideal for method screening. Employing traditional bonding technology and a unique endcapping technique, it minimizes the activity of silanol groups on the filler surface, resulting in superior chromatographic peak shapes for analytes. This column is widely utilized in the analysis and detection of samples from various fields, including pharmaceuticals, traditional Chinese medicine, food, and the environment.

The Alphasil VC-C18AQ column is a reverse-phase C18 column that incorporates polar groups through a polar embedding approach. In addition to maintaining the hydrophobic retention properties of the C18 bonded phase, it introduces additional polar interactions, enhancing the retention and separation selectivity of polar compounds. This modification also improves the surface wettability of the filler, enabling it to tolerate 100% aqueous phases. As a result, it is particularly suitable for the separation of polyhydroxylated polar compounds such as flavonoids and ginsenosides.

The Alphasil VC-HILIC column is a column bonded with diol groups, featuring a neutral surface that relies primarily on differences in compound polarity for separation. The VC-HILIC bonded phase is stable and resists column bleeding, enabling it to accommodate both hydrophilic interaction chromatography and normal-phase chromatography modes simultaneously. This versatility makes it widely applicable for the separation of polar compounds.

Alphasil XD Columns

The Alphasil XD-C18AQ column is a polar reverse-phase C18 column that employs a unique "polar copolymerization" technique, simultaneously bonding both C18 and polar groups. This design not only enhances the retention of polar compounds but also balances the retention and separation of non-polar compounds. It can tolerate 100% aqueous phases, making it suitable for the analysis of small molecular acids.

The Alphasil XD-C18CH is a polar reverse-phase C18 column featuring a positively charged surface modification. By introducing positive charges onto the silica surface, it effectively shields the secondary interactions between basic compounds and surface silanol groups, thereby improving the peak shapes of basic compounds. This column is particularly well-suited for the analysis of basic compounds, such as aconitine, ephedrine, or tricyclic antidepressants.

The Alphasil XD-Ph column utilizes a three-point bonding technique to attach phenyl groups to the silica surface, providing both hydrophobic and π-π interactions. This makes it suitable for the analysis and separation of moderately polar and aromatic compounds.

Alphasil S Columns

The Alphasil S series of columns represents customized products tailored specifically for individual separation targets, offering dedicated solutions for a particular sample or research project.

The Alphasil S-PFP column incorporates pentafluorophenyl groups, which provide dipole-dipole, π-π, and hydrophobic interactions simultaneously. This column is well-suited for the analysis of aromatic and conjugated compounds, with widespread applications in the separation of isomers.

The Alphasil S-AAA column is a C18 column specifically developed for amino acid analysis. Its unique bonded phase enables excellent separation performance for all 18 amino acids, with good reproducibility between batches and a long column lifetime. This column is widely used in the analysis of amino acids.

The Alphasil S-AC Pigment-Specific Column is a reverse-phase C18 column tailored for the analysis of synthetic colorants. Its unique bonding technology ensures superior separation performance for 11 types of synthetic colorants and exhibits exceptional durability when measuring synthetic colorants in complex matrices. This dedicated column boasts good reproducibility between batches and a long column lifetime, making it widely applicable for the analysis of various synthetic colorants.

The Alphasil XAqua C18 Calculus Bovis Column is a chromatography column specifically developed for the analysis of compounds in artificial calculus bovis. It inherits the excellent properties of the Alphasil, utilizing ultrapure spherical silica gel as its matrix. This column is meticulously crafted through the adoption of unique silica gel surface bonding technology and chromatography column filling processes, resulting in outstanding performance in terms of selectivity, stability, and other chromatographic properties.

Alphasil S-Xion is a zwitterionic hydrophilic interaction liquid chromatography (HILIC) column that significantly enhances the hydrophilicity of the packing material surface by bonding zwitterionic groups to the silica surface. This design enables the column to substantially reduce the proportion of organic phase in the initial mobile phase, thereby effectively improving the solubility of samples in the initial mobile phase.